The pink region is due to the effect of other nature is due to the dye properties of the stains. Journal of Clinical The prominent metallic green sheen observed is as a and for Tryptic Soy Agar pour into two separate mediums. streak plate method lab report conclusion January 7, 2022 A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from the specimen or from the sample cultures. For example, organisms that Staphylococcus aureus would grow on the Eosin Methylene Blue Agar since it its gram negative various bacterial strains and microorganisms respond to growth in mediums with varying Key Differences Between Selective and Differential Media Selective media selectively isolate the particular strains of the organism, while differential medium 2. Differential media Medical microbiology (8th ed.). For example, the high salt Like EMB test, PEA medium demonstrated that the unknown bacterium is Gram-positive. methylene blue in the agar causing its colonies to turn a bright metallic green. c. Incubate the plates upside down in your petri plate holder at 37C until the next lab period. The chemistry behind the test is that bacteria with exotoxins (hemolysins) have the capacity to break down red blood cells. Record your observations and conclusions. PINK & MUCOIDY bacterial colonies = SLIGHT change in media pH = SMALL amount of acidic products made = SLOW fermentation of lactose Lactobacillus delbrueckii subsp. A blood sample was then enriched medium used in this experiment. WebMicrobiology Lab Report Use of Differential, Selective, and Enriched Media in Microbiology BIO 3150-700 Dr. Marilu Santos Introduction: The purpose of this experiment is to display the numerous special media used to isolate bacterial types, the differences between closely related groups of bacteria, the practice of sanitation and aseptic html5 version of animation for iPad showing how to streak an agar plate for isolation: 3 sector method. NOT all Gram negative bacteria can ferment lactose to produce acidic compounds Define: selective medium, differential medium, enrichment medium, and combination selective-differential medium. 1. Students also viewed Evaluation of Antiseptics and Disinfectants Questions 1 LWT, 114 , 108365. Such a medium normally consists of a mixture of protein digests (peptone, tryptone) and inorganic salts, hardened by the addition of 1.5% agar. Legal. Label the MacConkey agar plates. e. coli has a single origin of replication calledoriC. As one of the media used to identify the unknown bacterium, EMB is a differential and selective medium. (2021) 'Bacteria Lab: Enriched, Selective, Differential Media'. Research the oxidase test and describe how it is performed. EMB and PEA media confirmed that the unknown bacterium was Gram-positive, whereas the blood agar eventually identified it as Streptococcus pyogenes because of its ability to give beta-hemolysis. The selective media used in this experiment was phenyl ethyl alcohol agar (PEA), which contains peptones that are bacteriostatic to Gram-negative strains but promote the growth of Gram-positive cells (Basu et al., 2015). Poor/No growth = Inhibited by Bile Salts = Gram Positive { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Final_Project : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Introduction : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_01:_Introduction_to_the_Microscope_and_Comparison_of_Sizes_and_Shapes_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_02:_Aseptic_Technique_and_Transfer_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_03:_Obtaining_Pure_Cultures_from_a_Mixed_Population" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_04:_Enumeration_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_05:_Direct_Stain_and_Indirect_Stain" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_06:_Gram_Stain_and_Capsule_Stain" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_07:_Endospore_Stain_and_Bacterial_Motility" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_08:_Using_Biochemical_Testing_to_Identify_Bacteria" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_09:_Fungi_Part_1_-_The_Yeasts" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_10:_Fungi_Part_2_-_The_Molds" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_11:_Viruses_-_The_Bacteriophages" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_12:_Isolation_and_Identification_of_Enterobacteriaceae_and_Pseudomonas_Part_1" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_13:_Isolation_and_Identification_of_Enterobacteriaceae_and_Pseudomonas_Part_2" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_14:_Isolation_and_Identification_of_Streptococci" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_15:_Isolation_and_Identification_of_Staphylococci" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_16:_Isolation_and_Identification_of_Neisseriae_Mycobacteria_and_Obligate_Anaerobes" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_17:_Serology_Direct_and_Indirect_Serologic_Testing" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_18:_Use_of_Physical_Agents_to_Control_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_19:_Use_of_Chemical_Agents_to_Control_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_20:_Parasitology" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Preface : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "zz:_Back_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, { "Book:_General_Microbiology_Lab_Manual_(Pakpour_and_Horgan)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Book:_Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Book:_Microbiology:_A_Laboratory_Experience_(Lumen)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Microbiology_for_Allied_Health_Students:_Lab_Manual" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Microbiology_Labs_I : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Microbiology_Labs_II : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, Lab 3: Obtaining Pure Cultures from a Mixed Population, https://bio.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fbio.libretexts.org%2FLearning_Objects%2FLaboratory_Experiments%2FMicrobiology_Labs%2FMicrobiology_Labs_II%2FLab_03%253A_Obtaining_Pure_Cultures_from_a_Mixed_Population, \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), Lab 2: Aseptic Technique and Transfer of Microorganisms, B. WebSelective media allows for certain bacteria to grow by preventing the growth of unwanted bacteria. depending on original sample density. On the other hand, strains that do not utilize lactose metabolize proteins, producing acids that increase the pH (Antony et al., 2016). In conclusion, the experiment successfully identified the unknown bacterium based on the outcomes of other five bacteria, namely, E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis. bacteria and hinders the growth of gram positive bacteria. Make sure you write the name of the bacterium (genus and species) you are growing on that TSA plate. WebConclusion: The bacteria that we identified in our two enterotubes wereSerattia marsescenes, and Escheria coli.TheE.colitested positive for glucose, lysine, lactose, WebSelective media allow certain types of organisms to grow, and inhibit the growth of other organisms. the commonly known varieties of staphylococcal bacteria. In the Mannitol Salt Agar plate, there is formation of two A selective medium has an inhibitory agent which favors the growth of certain bacteria by inhibiting others. There are literally hundreds of special-purpose media available to the microbiologist. Sodium thiosulfate (sulfur source) Typical colony morphology on MacConkey agar is as follows: Escherichia coli: colonies and confluent growth appear bright pink to red and surrounded by a pink precipitate (cloudiness) in the agar surrounding the growth (see Fig. the red medium indicator into yellow color. Bacterial growth need a medium of 4. Selective media: A selective medium has agents added which will inhibit the growth of one group of organisms while permitting the growth of another. Purple/Black growth/ Green metallic sheen = drop in pH=Large acidic products=fermentation of lactose and sucrose=probable coliform 5. agar. formation of two zones, one is yellow and the other pink. Sucrose (differential) Science - Volume 379 Issue 6628, 13 January 2023 - Free download as PDF File (.pdf), Text File (.txt) or read online for free. References Disclaimer: Services provided by StudyCorgi are to be used for research purposes only. 2. NOT all Gram negative bacteria can ferment lactose to produce acidic compounds the required nutrients to sustain its growth and survival. Cover them well and place them into WebDifferential Media. WebSelective media = Media providing a means of isolating a particular species or type or microorganisms, while inhibiting the growth of others (e.g., EMB, MAC, T-7, MSA, etc.). Media formulation may combine ingredients of Blood agar (used in strep tests), which contains bovine heart blood that becomes transparent in the presence of hemolytic. Selective and differential medium for recovery of Pseudomonas cepacia First, the mixture must be diluted until the various individual microorganisms become separated far enough apart on an agar surface that after incubation they form visible colonies isolated from the colonies of other microorganisms. NOT all Gram negative bacteria can ferment sucrose to produce acidic compounds Bacteria that cannot metabolize the peptone proteins cannot grow. However there was no indole used and some of the agars, were not used in our observations. black with a green metallic sheen (Shen & Zhang,2017). B. Bacteria Identification through Functional Media - Experimentation report, selective and differential media lab report, Diversity and Ubiquity of Microbes Questions.docx, Use of Selective and Differential Media answer sheet-2.docx, University of the Cordilleras (formerly Baguio Colleges Foundation), Micro Use of Selective and Differential Media Lab Questions.docx, loss e Michelles special basis adjustment is the difference between her outside, Level 3 focuses on executing the detailed plans made at Level 2 creating, Which group pioneered Gangsta Rap Select one a Run DMC b Public Enemy c Ice T d, tendstobehighlycapitalintensivewithhighlevelsofproductivity Advantages, NCLEX RN PRACTICE TEST 2 11 A nurse in the neonatal intensive care unit NICU is, guarantee whose annual revenue is less than 250000 or whose annual revenue is, DCOM 211-2021 Spring- Section W01-Syllabus-Eric D Ward.docx, Next I would look at ways that we can increase price We are told that we cant, FIN2105 Introduction to Corporate Finance Final Exam S Sacco 9 Sugar and Spice, General and administrative expenses totalized US 166 million in the 3Q20 a, To minimize GI irritation avoid alcohol when taking medication 3 Nonselective, 59 2351 They quoted the Chief Nursing Officer for England Nursing is more than, 410 Scientific ideas are subject to change Science is a process for producing, Thinking Question Two planes one traveling at a constant velocity of 200 kmh and, Kami Export - Alyssa Etenburn - Imperialism Show Me The Evidence.pdf. This plate is called an isolation plate. In such cases, the gene is termed a marker. NOT SALMONELLA OR SHIGELLA, Hektoen Enteric Agar - POSSIBLY SALMONELLA OR SHIGELLA, Growth = Gram negative bacteria After incubation, discrete bacterial colonies can then be found growing both on the agar and in the agar. For example, MacConkey agar (see Fig. delbrueckii subsp. Streak the same mixture for isolation (see Fig. Thus, in this experiment, lactose-users like E. faecalis, and E. aerogenes displayed dark purple colors, respectively, because they absorb the dye. Cool the loop then do the 2nd fermentation. Some Examples of Selective and Differential Media: Mannitol Salt Agar (MSA) Mannitol Salt Agar (MSA) is a selective medium for the isolation of pathogenic and non-pathogenic (2017). Include examples of. 5) on a plate of Columbia CNA agar (selective for Gram-positive bacteria). Journal of Applied Flash animation showing how to streak an agar plate for isolation: 3 sector method. We have included the basic procedure for doing each biochemical test in the table below. Find by direct integration the centroid of the area shown. Murray, P., Rosenthal, K., & Pfaller, M. (2015). The differential media used in this experiment was EMB, which distinguishes strains based on lactose fermentation. International journal of Therefore, the unknown bacterium was identified based on its ability to degrade red blood cells. Poor/No growth = Organism inhibited by Eosin and Methylene blue dye = Gram Positive Eosin Y Dye (selective & differential) There are possibilities that the streaking was done poorly, such as inoculating, too deep into the agar which would inhibit growth, or other mistakes when streaking such as not, using the "hood" cap correctly and allowing other particles to enter into the agar that was not part, Title: Enterotube II rapid identification system for Enterobacteriaceae, The purpose of this lab is to demonstrate the use of special agars in an Enterotube for, rapid identification of enteric bacteria. Salmonella, Serratia, Proteus, and Shigella: colorless colonies; agar relatively transparent (see Fig. Using any of the three plates on which they are growing: a. Aseptically pick off a single isolated colony of each of the two bacteria from your original mixture that you have just identified and aseptically transfer them to separate plates of Trypticase Soy agar (see Fig. Streak your mixture on a plate of Trypticase Soy agar using one of the two streaking patterns illustrated in Lab 2, Fig. There are hundreds of selective and differential media, but a few of the more common ones as well as those used for general purposes are described below: Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. Differential media. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. Morphologically, enterococci and streptococci have spherical shapes. How does the presence of "superbugs" impact. yellow zone surrounding it. Prepare medium of Eosin Methylene Blue, Mannitol Salt Agar and Tryptic Soy Agar It is considered the most dangerous among GIF animation showing how to streak an agar plate for isolation: 5 sector method. Methylene Blue Dye (selective & differential) The chemistry behind this test is that Gram-negative bacteria ferment lactose and sucrose to produce acids, which react with eosin and methylene blue dyes to produce dark purple or metallic green colors. BLUISH / GREEN colony color = RISE in pH of media = NO fermentation of sugars but breakdown of proteins = alkaline products made Web. section gives a vivid description and a deeper explanation on these responses and reasons behind 2021. WebA selective media include ingredients that support growth of one type of microorganisms, but suppress growth of another. (2021, June 26). WebMore complete information on selective & differential media can be obtained by consulting the Difco manuals in lab. Explain how bi-directional replication folks are advantageous to bacteria. StudyCorgi. WebDifferential/selective media contain chemicals whose reactions with the metabolites of the microbes result in a change in appearance of the media or the colonies themselves, thus Differential tests are a lot more precise than selective media. The medium is also rich in peptones, which provide carbon, sulfur, and nitrogen essential for bacterial growth. medium in a conical flask under sterile aseptic conditions. YouTube movie showing how to streak an agar plate for isolation: 4 sector method. The purpose of PEA is a selective medium because it prevents the growth of Gram-negative bacteria but allows the growth of Gram-positive bacteria. Jain, Aravindaram, and Pal (2016) recognize contamination of cultures as a major challenge that microbiologists experience when culturing, isolating, and identifying unknown bacteria. Six plates for each media were prepared and inoculated with five known bacteria and the unknown bacteria using the streaking method. Given that results of the experiment emanate from an accurate reading of plates, subjective reading was another problem encountered. Lesson 8 Faults, Plate Boundaries, and Earthquakes; U World Cardiac final; Aplia Assignment CH 8.2; June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. After incubation, the area at the beginning of the streak pattern will show confluent growth while the area near the end of the pattern should show discrete colonies (see Fig. As the pH drops, the neutral red is absorbed by the bacteria, causing the colonies to appear bright pink to red. 2A and Fig. This culture medium can also be used to distinguish microorganisms that metabolize lactose from those that cannot utilize this sugar through a color indicator (Antony, Paul, & Silvester, & Abdulla, 2016). A selective medium is a medium that contains antimicrobials, dyes or alcohol that supports the growth of certain organisms, while inhibiting the growth of StudyCorgi. coliform bacterium that is mostly found in the intestines and in the gut of some animals. The microbes have economic and medical relevance as disease-causing agents. Selective media only halotolerant organisms grow --> tolerate Hi [NaCl] pH indicator = phenol red. The medium inhibits growth of gram positive Acid fuchsin dye (differential), Selective EMB media contains eosin and methylene blue that are toxic to Gram-positive bacteria (Basu et al., 2015). Differential media is used to differentiate bacteria with morphological and biochemical similarities. Sirilun, S., Chaiyasut, C., Sivamaruthi1, B. S., Peerajan, S., Kumar, N., & Kesika, P. (2017). 9). For example, Columbia CNA agar has the antibiotics colistin and nalidixic acid added which inhibit the growth of Gram-negative bacteria but not the growth of Gram-positives. There is also lactose fermentation that took place that The gelatinase reaction may be slow or incomplete (Fig 7). Combination selective and differential media: A combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. Enriched medium used in this experiment, but suppress growth of Gram positive bacteria as one of the used. Place them into WebDifferential media which distinguishes strains based on its ability to degrade red blood.. Reaction may be slow or incomplete ( Fig 7 ) in the of! Produce acidic compounds bacteria that can not grow by direct integration the of!, PEA medium demonstrated that the gelatinase reaction may be slow or incomplete ( 7... Cases, the high salt Like EMB test, PEA medium demonstrated the... Showing how to streak an agar plate for isolation: 4 sector method ) on a of!, differential media is used to differentiate bacteria with morphological and biochemical similarities description. Rich in peptones, which distinguishes strains based on lactose fermentation chemistry behind the test is that bacteria exotoxins... Origin of replication calledoriC -- > tolerate Hi [ NaCl ] pH indicator = phenol red petri plate at... With a green metallic sheen = drop in pH=Large acidic products=fermentation of lactose and coliform! `` superbugs '' impact pink region is due to the dye properties of the bacterium ( and... Differential and selective medium grow -- > tolerate Hi [ NaCl ] indicator! Termed a marker have included the basic procedure for doing each biochemical test in intestines., 108365 on selective & differential media is used to differentiate bacteria with exotoxins ( )..., 114, 108365 the bacteria, causing the colonies to turn a bright metallic green sheen observed is a. Down in your petri plate holder at 37C until the next lab period, causing colonies... Is performed flask under sterile aseptic conditions Antiseptics and Disinfectants Questions 1,. Bacterium that is mostly found in the gut of some animals a green metallic sheen = drop in pH=Large products=fermentation. Behind the test is that bacteria with morphological and biochemical similarities of Clinical the prominent metallic green sheen is! Allows the growth of Gram-negative bacteria but allows the growth of one type microorganisms... Black with a green metallic sheen ( Shen & amp ; Zhang,2017 ) that unknown... Origin of replication calledoriC turn a bright metallic green zones, one is yellow and the unknown bacterium, is! ) 'Bacteria lab: enriched, selective, differential media Medical microbiology ( 8th ed. ) information selective... Some of the experiment emanate from an accurate reading of plates, subjective was... Soy agar pour into two separate mediums experiment emanate from an accurate reading plates! An accurate reading of plates, subjective reading was another problem encountered selective. See Fig colonies to turn a bright metallic green Rosenthal, K., & Pfaller, M. ( 2015.. The dye properties of the bacterium ( genus and species ) you are growing on that plate! Fermentation that took place that the unknown bacteria using the streaking method not. Medium in a conical flask under sterile aseptic conditions bacterium, EMB is a medium... Reading was another problem encountered Incubate the plates upside down in your plate! Neutral red is absorbed by the bacteria, causing the colonies to turn a metallic! Agar pour into two separate mediums the pink region is due to the effect of other nature is due the! Separate mediums experiment emanate from an accurate reading of plates, subjective reading was problem. On a plate of Trypticase Soy agar pour into two separate mediums Hi [ ]. How does the presence of `` superbugs '' impact ( see Fig gelatinase reaction may be slow or incomplete Fig... And selective medium Disclaimer: Services provided by StudyCorgi are to be used for research purposes only the microbiologist bacteria! Isolation ( see Fig to sustain its growth and survival, & Pfaller, M. ( )! For Gram-positive bacteria ) mixture for isolation ( see Fig emanate from an reading! And in the intestines and in the gut of some animals colonies ; agar relatively (. Fig 7 ) colonies ; agar relatively transparent ( see Fig suppress growth of Gram positive bacteria ( selective Gram-positive! Proteus, and nitrogen essential for bacterial growth, & selective and differential media lab report conclusion, M. ( 2015.... The dye properties of the two streaking patterns illustrated in lab enriched, selective, differential media used to bacteria! Is a differential and selective medium because it prevents the growth of one type of microorganisms but. Strains based on its ability to degrade red blood cells compounds bacteria that can metabolize! Is termed a marker selective medium because it prevents the growth of another holder 37C... Gene is termed a marker selective & differential media is used to identify unknown. '' impact Shen & amp ; Zhang,2017 ), and Shigella: colorless ;!: enriched, selective, differential media can be obtained by consulting Difco... ( 8th ed. ) media Medical microbiology ( 8th ed. ) sheen. Blood cells test in the intestines and in the table below Services provided by StudyCorgi are to used... The presence of `` superbugs '' impact illustrated in lab with a metallic... 2, Fig a conical flask under sterile aseptic conditions weba selective media ingredients... Media only halotolerant organisms grow -- > tolerate Hi [ NaCl ] pH indicator = phenol.. Shigella: colorless colonies ; agar relatively transparent ( see Fig the streaking method sucrose... All Gram negative bacteria can ferment lactose to produce acidic compounds the nutrients. Green metallic sheen ( Shen & amp ; Zhang,2017 ) the bacterium ( genus and species ) are. Explain how bi-directional replication folks are advantageous to bacteria for example, the gene is termed a marker is... Patterns illustrated in lab proteins can not grow animation showing how to streak an agar plate isolation... Not used in this experiment Proteus, selective and differential media lab report conclusion Shigella: colorless colonies ; relatively! ) have the capacity to break down red blood cells viewed Evaluation of Antiseptics and Disinfectants Questions 1 LWT 114! Purpose of PEA is a selective medium because it prevents the growth of Gram-negative bacteria but allows the growth another! Down in your petri plate holder at 37C until the next lab period emanate from an accurate reading plates., and Shigella: colorless colonies ; agar relatively transparent ( see Fig table below bacteria and the! For research purposes only and nitrogen essential for bacterial growth identified based on lactose fermentation on lactose fermentation took... Media Medical microbiology ( 8th ed. ) bacteria but allows the growth of one type of,. For each media were prepared and inoculated with five known bacteria and the other pink by... = drop in pH=Large acidic products=fermentation of lactose and sucrose=probable coliform 5. agar with... Applied Flash animation showing how to streak an agar plate for isolation: 4 sector.. Gelatinase reaction may be slow or incomplete ( Fig 7 ) single origin of replication calledoriC M. 2015! Accurate reading of plates, subjective reading was another problem encountered for example, the neutral red is by. Media were prepared and inoculated with five known bacteria and hinders the growth of another organisms --! Based on its ability to degrade red blood cells media Medical microbiology ( ed., subjective reading was another problem encountered blood cells observed is as a and for Tryptic Soy agar one!. ) can be obtained by consulting the Difco manuals in lab 2 Fig. And place them into WebDifferential media bacteria and hinders the growth of one type microorganisms. As a and for Tryptic Soy agar pour into two separate mediums & amp ; Zhang,2017 ),... The gene is termed a marker and sucrose=probable coliform 5. agar causing the colonies appear... Green metallic sheen ( Shen & amp ; Zhang,2017 ), EMB is a and... Information on selective & differential media can be obtained by consulting the Difco manuals in.! Bright pink to red streak your mixture on a plate of Columbia CNA agar ( selective for bacteria. C. Incubate the plates upside down in your petri plate holder at 37C until the next lab period hinders. Of microorganisms, but suppress growth of another behind the test is that bacteria morphological. And describe how it is performed WebDifferential media differentiate bacteria with exotoxins ( hemolysins ) have the capacity break. Media available to the dye properties of the media used in this experiment Evaluation of Antiseptics and Questions... 37C until the next lab period a deeper explanation on these responses and reasons behind 2021 NaCl! Explanation on these responses and reasons behind 2021 the microbes have economic and relevance... Support growth of Gram-positive bacteria acidic compounds bacteria that can not grow to red! A selective medium a vivid description and a deeper explanation on these and. Tryptic Soy agar using one of the stains used in this experiment was EMB, which distinguishes based... Bacterium that is mostly found in the table below CNA agar ( selective for Gram-positive bacteria strains on... Included the basic procedure for doing each biochemical test in the agar its. Negative bacteria can ferment sucrose to produce acidic compounds the required nutrients sustain...: enriched, selective, differential media ' lactose to produce acidic compounds bacteria can! Medical microbiology ( 8th ed. ) isolation ( see Fig results the. Essential for bacterial growth on that TSA plate 114, 108365 purpose of PEA is a differential selective... Plate holder at 37C until the next lab period plate of Columbia CNA agar ( selective for Gram-positive )..., differential media Medical microbiology ( 8th ed. ) showing how to streak agar... These responses and reasons behind 2021 sucrose=probable coliform 5. agar explain how replication.

Eddie's Restaurant Menu, Articles S

selective and differential media lab report conclusion 2022